Polynucleotide Synthesis

Polynucleotide synthesis refers to combining nucleotides to form new sequences. In the early 1960s, researchers were able to synthesize a bacterial transfer RNA for tyrosine. Now we have machines that can synthesize polynucleotides of a predetermined sequence up to 75 nucleotides long. If you plan your experiment carefully, you can synthesize many of these predetermined sequences and join them together to make a longer sequence. Using this technique, researchers can modify genes, insert them into the host and watch the responses. Imagine the possibilities of growing replacement chromosomes instead of splicing repaired genes. Exciting isn't it?